ZitatAlles anzeigenA phylogenetic study of the species in Boletus sect. Boletus was undertaken using the molecular markers
ITS1-5.8S-ITS2 and GAPdH. Four well-supported lineages, one comprising Boletus edulis s.l., the others referring to
B. aereus, B. reticulatus and B. pinophilus have been distinguished. The ML and MP trees of ITS showed remarkably
low resolution within the B. edulis clade, and conï¬rmed earlier published results, despite the use of samples from a
wider geographical area and different hosts. The results of GAPdH demonstrate clearly that this low resolution must
be ascribed to a low genetic variability with the B. edulis clade, and make clear that morphological and ecological
characters have been overestimated within this species complex. Boletus edulis is therefore deï¬ned as a variable spe-
cies with a wide morphological, ecological and geographic range, and includes several speciï¬c and subspeciï¬c taxa
described in the literature (e.g. B. betulicola, B. persoonii, B. quercicola and B. venturii). Three other European species
(B. aereus, B. pinophilus and B. reticulatus) are well delimited species based on morphology and our genetic data.
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