Cortinarius sp. ? under Cistus (and Thymus) from calcareous soil

Three specimens under Cistus and Thymus. Pileus 5-6cm, pale yellow, dry, though, glabrous-polished (when cleaned from debris), very bitter taste, no volva or ring or veil (at least not distinct), spore print light cinnamon brown, lamella adnate, stem shiny pale brown. Spores pip-shaped, surface rough (bumpy). IKI inamyloid (cell wall appears dextroid?!).

Am I on the right track if I consider Gymnopilus sp. ?

That would be the first Cortinarius sp. for the country 😍

Dear all, I am very excited about this find. Many mycologist thrived to find the first Cortinarius from Malta. I spent the whole afternoon checking the microscopical features yesterday for a determination, and here i find some suggestions already.

T aste : It was incredibly bitter, I had to spit it out the small nibble I took.

Cuticle: The debris of soil and leaves stuck on the cuticle indicate that it was viscous when young. The stipe seem to be not. The colour was pale yellow, like straw changing to light cinnamon with age... so maybe it was paler when young.

Reactions: Iodine no reaction on cap or flesh; 4% KOH - slow reactions, slightly dark brown with a hint of olive-green hue on cap after 20 mins. Faster reaction with 10% KOH and somewhat more pronounced olive-green hue (tested on drying cap); 4% KOH on flesh gave a pale greyish-beige colour.

Lamella: Adnate to sub-decurrent, convex, cinnamon brown but it turns purplish-brown when bruised of wettened (eg in mount). I don't know if this is characteristic. Edge indifferent from face.

Basidia: Rectangular-clavate, with a subtruncate apex, flexuose (curved), many with few to several oil bodies, in my opinion mostly 2-sterigmate, some 3- a few 4-(?), sterigmata 3 um, horn-shaped, length 24-29um.

Basidia: Rectangular-clavate, with a subtruncate apex, flexuose (curved), many with few to several oil bodies, in my opinion mostly 2-sterigmate, some 3- a few 4- (?), Sterigmata 3 um, horn- shaped, length 23-29 µm.

Cheilocystidia: If I have observed correctly, they are quite indistinct as expected for this genus, cylindrical, sometimes curved, do not stain very well in Congo red, rare. Same size of basidia (maybe I have observed basidiomes). Pleurocystidia not observed (or rare and missed)

Spores: Pip shaped, subglobular to broadly ovate, heavily warted showing shadow-lines under the x100 oil immersion, apiculum distinct, central, elongated. Germ pore absent, but there is a discontinuous wall at the apiculum (I don't think it can be considered a sa germ pore?!). Quite variable in size and sometimes shape (few are elongated and longer (+2.5 um above the average).

Spores: Pip shaped, subglobular to broadly ovate, heavily waited showing shadow-lines under the x100 oil immersion, apiculum distinct, central, elongated. Germ pore absent, but there is a discontinuous wall at the apiculum (I don't think it can be considered a sa germ pore ?!). Quite variable in size and sometimes shape , few are elongated and longer (+2.5 um above the average length). Inamyloid but wall seems to be dextrinoid.

Pileipellis: two layers (maybe only the upper is the true pellis). The outermost (suprapellis) a trichoderm of interwoven curved/ worm-shaped hyphae, 3-6um wide with rounded apices (very slightly inflated) and several clamp junctions.

Below lies the subpellis composed of wider and shorter hyphae, tubular or cylindrical in shape, with less frequent clamp junctions, 10-15(-17) um wide.

I see, so the infractus group can be confirmed at least! I wanted to add info (micro) perhaps it can lead to a narrower selection within the group. Ok then.

Who do you think would be interested to follow a DNA analysis?

Is a dry specimen enough to sequence or it has to be subcultured on special agar?

Or to put in different ways, what can I do to sequence it? (with my own budget)

Thanks.

Lovely!!! Thank you and I agree re sequencing - I check both and maybe start from the Estonian but 30 Eur is not much. I may submit a larger sample size (I have a Cosmospora that is really hard to determine) to the spanish Lab later.

Im happy in this forum

Dear Uwe, I just wrote this morning to Unite / Estonia and I see what they are gonna tell me. I will keep you informed with everything. Thank you for your help and I see no objection on sharing the sequence with serious colleagues like your kind self. I have no experience with the sequencing of fungi so I am glad to read your offer of helping.

Apart this I have other fungi that are crying for sequencing such as a Cosmospora (?) sp. [ Cosmospora species , Central Mediterranean region - Forum ASCOFrance] and a Talaromyces growing specifically on Washingtonia seeds. [ https://mushroomobserver.org/262509 ]

Zitat von Cortinarius

Hi Steve,

I do my sequencing with Alfalab.es , Pablo Alvarado has a lot of experience
You will get the results in about 3 weeks , which is quite OK

The price is about EUR 22,00 per sample.

If you like , you can share the sequence data with me, I can help with the interpretation

With rare species its sometimes tricky to interprete the results.

If you are interested contact my by Private Message
BR
Uwe

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Dear Uwe,

I herad nothing from Unite, so giving them the fair chance to reply, and they did not, I am OK to move on with you. This is the first time to send material for DNA sequencing, hence where should I send the sample to you UWE? - I send you PM as requested