Calycella citrina and a pale Calycella citrina ?

On a fallen small branch of an Olive tree, I spotted some tiny bright yellow ascomycetes which I rolled (ie the entire branchlet) in Aluminum foil and collected it home . Three days later I went to examine the branch and I found two ascomycetes now - (1) the yellow Calycella and (2) another population similar in size to it but gray to mouse gray. Are these some new growth of decolourised specimens of Calycella (because perhaps they where in the dark?)

and also, is C. citrina confirmed from these photos or there are closely related species to be considered (hence I need to run a full micro? )

Photos were taken with artificial light so some colour inaccuracies are there, but they are like highlighter yellow, bright. I tried to save some microwork, but i think they are different so I postpone for tomorrow. Would be great if C. sulphurea! Thanks andreas!

pilzforum.eu/attachment/363503/I have carried out the micro of the yellow asco. From some observations under the stereo, first impressions are that the pale ones are the same one more or less decolorized because some of them have a bit of yellow pigment in them. The atypical border is perhaps because they are dry ?!

The asci are dextrinoid or stain strongly in IKI except where there are the spores , otherwise inamyloid at the tips; 60-80um long and only 5 um wide. The elongated spores overlap each other in the ascus. The spores are interesting and maybe do not corresponding with C. citrina. They are narrowly fusiform, almost linear with four bands due to the presence of four evenly spaced guttulae. They measuring roughly 10-14 µm x 2-3 µm. They do not stain well in Congo red or IKI. I believe there is a central septum.

Flickriver: Searching for photos matching 'Bisporella sulfurina'

Zitat von mollisia

Zitat von Beorn

MoinMoin!

Is "Calycina sulphurea" synonym to "Bisporella claroflava"?

LG; Pablo.

Hello Pablo,

yepp.

I worte incorrect "sulphurea" but I meant "sulfurina". Bisporella sulfurina = Calycina sulfurina = Calycina claroflava

all the best,

Andreas

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I am lost in nomenclature : Bisporella sulfurina = Calycina sulfurina = Calycina claroflava = Calycella sulfurina ?

Dear Andreas, unfortunately, I could not photograph the poorly stained spores very well, but they had one septum. an elongated fusiform shape and usually 4 oil drops. I can confirm that the asci were inamyloid at the tips. Another thing, the ascocarps were growing on bark of a dead olive branch and associated (or on) pyrenomycetes over the bark. All these are indicators for C. sulfurina from the small amount of research I did.

I used Lugol's Iodine solution - I don't know... but it works for me to stain the Asci tips and less dangerous. I do have Melzer's, so if crucial I can test the asci with it again.

I am very disappointed with my image qualities of thid fungus - sometimes I get good images and sometimes I don't. This did not stain very well. I photograph stuff from my Digital camera through the eyepiece. (my microscope is not trinocular ) .

Bisporella sulfurina? - Forum ASCOFrance