I have a question related to the cultivation of molds on media and selective media like PDA, MEA, OAT Agar, Czapek, etc. I have left some 25g of antibiotics (approx. 10g Augmentin, and 14g Moxiclav *). This was for human consumption but they are general antibiotics. I want to add them to my media to control bacterial colonies. [* both brands state that each 1g tablet is composed of 875g Amoxicillin + 125g Clavulanic Acid]
Q1: Can you suggest how much I should put, say in 250 ml of media during preparation.
Q2: Is it Ok to add when hot, or hot water destroys the antibiotic?
Q3: They expire end 2021 but do they really expire for in-vitro use?