Green-yellow Aspergillus

I have collected this Aspergillus from the fleshy mesocarp (and exocarp) of Iris seeds. I cultivated it and got this beautiful green colony with chlorine-yellow green hues. A quick look under the microscope and it was an Aspergillus sp. I had dense conidiophores under the microscope and nothing photogenic, but I try the scotch tape method tomorrow. I wonder if there is a specific aspergillus which gives the colour combination.

It was less colourful on Malt Ext Agar and very colourful on Czapek. I am cultivating it on Oat Meal Agar too just to check how it looks.

Sorry for the jumbled sequence - here are the agars in sequence of position in the abovepost

1. PDA

2. Czapek

3. Malt Extract

4. Malt Extract

5. Czapek

I attach here reverse plate of PDA

OK, I will do that right now. Microfungi always astonish me under the microscope but they are often disappointing re identification esp Aspergillus and gulp! Penicillium! I don't have DG18 I see from where I can buy it (do you know some good online shop that the prices are for home mycologists !

Zitat von Florian1986

Hi Steve,

i am afraid that species determination in this genus is not possible without molecular data. Currently there are more than 450 species in this genus and i am sure, that there are still a lot of species, which are undescribed yet.

Best regards,

Florian

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Yeah I was afraid from getting this reply. Actually, I am drying some agars maybe I sent them to Pablo.

Here are some microscopical data.

The heads are in my opinion une-seriate, but better confirm from the images. The spores are spinulose, 5-6 um, they do not change colour in KOH. The conidiophores are thick and widen close to the head and there is some 'wrinkling ridges' just below the head (characteristic ???) and covered with dense small warts. Phialides are about 12-15 um long with tapering tips. Conidiospores are produced in long chains and are difficult to separate from the heads. The heads are quite strong, I could not break one to get loose the phialides!

According to my simple book (but I love its keys) this would be A. flavus (easy peasy as that ? I don't think so !) but I am seeing colonies of A. parasiticus offer a closer match!

Thanks, I selected this mold for sequencing - I keep u posted - hope it works out.

1. Dried agar at 35-39 C

2. Cut agar into small pieces (quarters)

3. Placed in an aluminum pocket

4. Then in a paper pouch

5. Placed paper pouched in a bag with silica gel

P..s. in the agar I added antibiotics to prevent bacterial growths (it helps a lot) hope this would not interfere with the sequencing!