Dear friends,
Today I received several results of ITS sequencing of moulds. This post is specifically about a Cladosporium where the same colony was subculture into two different plates (U11a and U11b), and I submitted both for sequencing. The following results are received (I have fasta / ab1 files for both):
U11a = ok, 100% Cladosporium tenuissimum MK513837, C. cladosporioides MF475952, Hanseniaspora uvarum MG250496
U11b = ok, 100% Cladosporium cladosporioides MT466517,C.cubuliforme MT312738
So I have a tie (undecided) between C.cladosporioides and Cladosporium tenuissimum.
How can I decide which is the one???
1. Morphological examination (can it tell the difference between the two) ?
2. Do other molecular tests (LSU ...???)
3. Edit the sequences and repeat the phylogeny on BLAST/MEga-X
4. Plate on different media?
I prefer the cheaper options (hence avoid doing more tests! unless there are other cheaper options!)
I am an eager amateur