Beiträge von Steve_mt

Zitat von Steve_mt

Dear Oehrling and Michael, thank you for your replies.

Ring or not, I am not sure because I do not see a scar on the stem, but you could be right.

I reckon microscopy is required at this stage and I have marked that I have an exsiccatum for SM-552. I report findings over the weekend.

Thank you

LG / FG

Stephen

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So finally I had time to examine the spores and gills of an exsiccatum which was not in very good shape (larvae destroyed physically part of the specimen. Here are some notes:

The gills turned beige-brown with age

Spore print white

Spore deposit/production abundant

Cystidia generally absent (but check image of a possible finding of a cheilocystidium)

spores broadly ovate or shield like with apiculum and distinct germ pore

Sizes:

(7.5) 7.8 - 9.3 (10.2) × (5) 5.3 - 6.1 (6.6) µm //

Me = 8.5 × 5.8 µm; Qe = 1.5 ; Ve = 147 µm3

Ring may be present and got detached and hanging at the rim during basidiocarp growth

I am thinking on Leucoagaricus, specifically L. leucothites ?

>I cannot upload pics!!!<

.... wanted to add that I bought this

https://proficook-france.fr/en/proficook/37-deshydrateur-alimentaire-4-ebenex-proficook-pc-dr-1116-4006160501117.html

The lowest temperature is 40C and unfortunately my thermometer (external metal thermometer) indicates 44C eith lid closed. Removing the lid and raising some compartments, I get around 36-40C. Hope this is fine for molecular testing and cell conservation for microscopy.

I wonder if anyone uses this too

This topic is of interest also to me. At the moment I have been struggling in finding a cheap or free image stacking software mostly for myxo but also for micro-images and small mushrooms. Any suggestions ?

Question 2 is that I have an SLR and I can have an adapter so as to fit in the microscope trinocular head (an old 1980s AxioLAB Zeiss). Do you have experience if photos taken this way produce good quality images. I am currently taking pictures of my eyepiece from an expensive point and shoot camera. Its 4x optical zoom enlarges the image nicely and it is very fast.

With the SLR the shooting is a bit slow procedure as I assume one has to refocus and adjust settings between live view and switching to shooting mode. Also I loose the ocular micrometer option.

Dear Oehrling and Michael, thank you for your replies.

Ring or not, I am not sure because I do not see a scar on the stem, but you could be right.

I reckon microscopy is required at this stage and I have marked that I have an exsiccatum for SM-552. I report findings over the weekend.

Thank you

LG / FG

Stephen

I am trying to identify this species found under carob trees and some pine needles from a tree further apart, on damp humus. No partial veil and I don't see a vulva. 26/11/2020

Thanks, will do over the weekend.

spores, cheilo/pleuro, and pileipellis.

Thank you, we have the same thoughts on this. I'll check the micro shortly. I was wondering if it is too small for L. subincarnata?

Thanks for replying

I have another Lepiota to show you. No microscopy for the time-being but specimen are being dried. Three specimens growing under oak tree (Quercus ilex) although Pine trees are also present not far. Specimen small, around 3cm tall with a cap about 2cm. Ring present but deteriorated with time. From my understanding, the stipe darkens to reddish brown with age that at one point when at home, I thought I might have gathered two different species, but on seeing photos in situ I believe they are all the same species. Margin pleated. Very elegant the tufts of veilar remains, toffee-brown in colour. Your opinions are welcomed on how to help to get an identification, unless molecular sequencein is the only way forward. Thanks!

Hello, rainy and cold season started in Malta and the mushrooms are punctual.

I was lucky to find something quite rare (for my eyes!) a small Lepiota with nice pattern of veilar remnants on the cap. I have dried a specimen for later microscopy, but at the moment I wish to have some clues. The fungus was about 2.5 cm tall and a cap of about 2 cm across. Scent indistinct or faint. It was growing in a mixed aforested area in a valley (sem-riparian) and the two trees present were Quercus ilex and Pinus halepensis. I have got an idea of L. subincarnata or because it is small, L. echinella.

I can carry microscopy after 5 days because I am away from my home Lab.

It has been decided at this hour during a meeting by JONEF members and we updated the deadline during this meeting to 10th Dec. It will be shown online soon, too.

Tnx

Dear myco friends,

I am part of a project called Jonef which is building a report for the conservation and protection of fungi based on two questionnaires.

Info on the project can be found here

Project JoNeF – Joint Network for wild Fungi | Network per la diversità micologica

The direct links for these questions are found here.

PART 1

Füllen Sie bitte dieses Formular aus

PART 2

Ausfüllen | JoNeF Questionnaire-base Survey (Part II)

Deadline 10 December.

It is an easy online data input.

Your participation is welcomed and important especially because we lack data from germany, poland (and spain).

Thank you

This is Biscogniauxia mediterranea on dead Quercus ilex tree.

Zitat von BlonBoah

Hello Steve,

so far I think the new system is even better than the old one!

But I understand what you mean.

By the way: Fuligo was not placed with Diderma but Mucilago with Didymium, but I could well imagine that Fuligo will soon belong to Physarum.

Kind regards

Noah

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Yes, sorry - bad memory - I meant that combination -> Mucilago into Didymium

Badhamia foliicola

Badhamia foliicola

Hermosa especie de myxomycete con un capilicio delicado y tridimensional, parte inferior de la esporoteca oscura por transparentarse las esporas de su…

www.flickr.com

For a long time the study of myxomycetes was my favourite because the classification and keys and genera were quite straightforward and there was not much interference from genetic disruptions. Hope that more or less, things in slime moulds remain like this. Having Fulgido absorbed in Diderma is acceptable but let us see what happens in Badhamia and Physarum and if the basics we learnt have to be erased with new molecular concepts that not always make straight sense to a sensual brain!

I have found this cluster of myxocarps aggregated in tight groups (but never in plasmidiocarps) on a decaying legume (fruit) of a carob tree. After sitting in the waiting list for months, today I decided to study this slime mould.

The myxocarps are about 0.5-0.9 mm across, seem spherical but a lateral view shows they have a conical blackish base and sometimes a cream-mustard pseudostipe, making some of the fruiting bodies have a pear-shape structure. Interestingly the lime on the peridium is not thick, rather vein-line with nodules scattered here and there and overall, the peridium is moderately irridiscent esp. in artificial (LED) light.

Now comes the hardest part of me - Badhamia or Physarum type of capillitium?????

Essentially, the structure is made of columnar lime with various Y-shaped particles but also with fine, hyaline threads from them. For me, it is more a Badhamoid capillitium, but not sure.

The spores are globular, spinulose-punctate with very tiny dot-like projections rather than warts of spines, evenly distributed without dark or pale patches or lines or irregular patches whatsoever. They measure (8.5)9.0-10.0(10.5) um across, rather homogenous.

In the end, I concluded it is Badhamia foliicola (second preference a Physarum cinerea) but I wait confirmations after I assign this name to the species.

Thank you

yes, truly beautiful species both as a singular myxocarp as you well explained, and also in groups, with those quite equidistant spaces between sporocarps making everything very pleasing to the eye.

Thank you for the update, something we need to get familiar with although the changes do not seem as drastic as in Fungi and to a less extent plants (although placing some snapdragons with same family of plantains is still something I am still digesting slowly)

About two weeks ago, I came across a strange coal-like fungus on the bark of a dead, unidentified tree, which had the texture and looks of a Daldinia, but it was flattened so I could not assign it as the two species known in Malta - D. concentric and D. raimundii. These have distinct semi-spherical or cushion-like fruiting bodies, with concentric rings, hence to exclude, but not sure if other Daldinia species that are flattened. I have now considered Biscogniauxia sp. but not sure which sp would be. I can revisit the population for microscopy and further examinations. The tree is not beech, maybe Quercus

Dear Raphael,

Many thanks for your feedback. Tomorrow I send the main corresponding author an email and see what I get. Despite DNA analysis via Blast and GenBank, it is not always assured your best-matched sequence has the correct name, hence studies like those you mentioned come useful. Thanks again!

Zitat von CH-Andy

Hello

Very interesting Lepiota in such small cases, I would also bring following Option in the inner choice -> Lepiota pseudolilacea

In the fourth picture the color is more black, are you Show two different types?

Best regards Andy

Edit, Chris was faster 😉

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Hello Andy, thank you for your suggestion too. I appreciate The fourth pic is the same specimen but not in natural light but under the stereomicroscope with badly assigned White Balance. I played a bit with the contrast too so ignore the colours in the 4th pic. Yet when i dried it, the colours darkened.

Zitat von chris77

Hi, this is pseudolilacea in my opinion

Chris

Edit: Here's a find from the last few weeks

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Thank you for your opinion, I was unaware about L. pseudolilacea (does it grow in this habitat too?). I admit that it resembles it a lot, although I would love to know the major distinction between the two sister species. I see if I can find some literature on my behalf !

Hi, I have sequenced some specimens of Pluteus, and would like to know if someone is working on this Genus and if my DNA sequences from Malta would be handy. I have sequenced this population with slightly yellowish stipe, and the LAB suggested Pluteus cf nanus. I am not sure if it is this species if it the name was not correct when the author of that sequence made a good or bad identification.

Anyway, I would love to hear your opinion on this Pluteus sp.

LG

Steve

This small Lepiota sp. was found on moss and grassy soil in a damp site over calcareous limestone. It was not in a very good state but I could see a brown-purplish ring which alerted me on L. lilacea. On age this darkened to blackish-purple-brown.

I have some microscopic data too if required. Spores scarce, could not find them

https://www.researchgate.net/publication/311517555_Polypores_and_genus_concepts_in_Phanerochaetaceae_Polyporales_Basidiomycota/figures?lo=1