The images are amazing. Do u have the monograph ? If not I can send
Beiträge von Steve_mt
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Thanks, Clavaria for the info.
I reworked the spores and the sizes are those below, making it a clear D. coprophila (spore size = mean 12.4 x 7.6)
(10.4) 11.8 - 13.2 (14.1) × (6.2) 6.9 - 8.2 (9.3) err
Q = (1.5) 1.51 - 1.8 (1.9) ; N=31
Me = 12.4 × 7.6 err ; Qe = 1.6 ; Ve = 381 err3
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Hello!
Clitocybe it is. I think it can remain within the variability of C. hobsonii!
The real name is "Clitopilus", not Clitocybe.
http://www.pilzflora-ehingen.d…ora/arthtml/chobsonii.php
VG Ingo W
Dear Ingo,
Yes I got tired yesterday - I corrected the names. I agree re hobsonii, yesterday night I found the differences between the two, mainly the spore length with hobsonii being: 7.5-8.5 and daamsii : 8.0-11.5 um. I really a merry-g0-round here but it is nice to learn. i will never forget the vertical ridges now
Thanks to all - I appreciate a lot
LG
Steve
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I abandoned C. hobsonii because of its smaller spore size. Also, the robust apiculum is something which mislead me. But yes, I agree about the longitudinal shadows of the spores (see a better image here!). Moreover, I went to check for the Cheilocystidia and could not find any -I think what I am seeing are basidioles. If I remember what I read in the afternoon, lack of cheilocystidia is a Clitocybe character.
Clitocybe it is. I think it can remain within the variability of C. hobsonii!
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Further research and applying the key on European species, I have landed on Crepidotus subverrucisporus , typically on calcareous habitats
https://www.mykoweb.com/Crepidotus/species/Crepidotus_subverrucisporus_subverrucisporus.html
C. variabilis has smaller spores (Sp 5.5-7.5 x 2.5-4 μm), and more narrow - subcylindrical
C. cesatti has more broad (subglobose) spores (Q. <1.4)
C. luteolus has yellow tinge (at the less)
C. lundellii* is closely related but it is said to have brownish colours when dry and not calcareous
So at the moment, this is Crepidotus subverrucisporus or C. lundellii - I guess I have to re-examine the cheilocystidia?
C. lundellii: https://www.hlasek.com/crepidotus_lundellii1en.html
* One of its synonyms is Crepidotus inhonestus !lol! - why this fungus is dishonest haha!
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I have given a quick check at the spores and I think you are right about Crepidotus - the spores are too large for C. hobsonii, and their shape, a bit like a lemon due to a distinct-swollen apiculus is also Crepidotus rather Clitocybe.
Spores:
(7) 7.1 - 8.3 (8.9) × (4) 4.1 - 4.8 (5.2) µm
Q = (1.5) 1.52 - 1.8 (2) ; N = 12
V = 61 - 98 (110) µm3
Me = 7.6 × 4.5 µm ; Qe = 1.7 ; Ve = 81 µm3
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I think you are correct (or Botryotinia) but it must be a species that does not have a branched conidiophore. I always associated Botrytis with a branched conidiophore (that is I have always seen B. cineraria). I see what I find in this genus with large spores. B. elliptica for example!.
Thanks for the moment !
Hi Steve,
compare with Botrytis sp.
best regards,
Thorben
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OMG there is also subcoprophila! How to tell the difference between the two? I have some microscopic data at hand, such as the spores.
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I forgot to add that I managed to get a pure colony on Sabourad Agar. It formed a white or light grey mycelium without quick fruiting bodies. These were formed after 1 week when the mycelium covered the whole plated and they were formed at the walls of the petri dish (drier regions ?), somewhat not that black and shorter. Now I have inoculated OAT, PDA and CZapek to see if it helps. Really wish to learn this microfungus. Chloridium is close but spores are larger 10-17 um long, 8-11 um wide
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Growing on the pulp of a fallen fruit of Hawthorn (Crataegus monogyna) was a dark mycelium with white heads which took my curiosity to investigate. Under the microscope I could see a simple, dark-coloured (blackish-brown) septate conidiophore (somewhat paler at the tips or undeveloped ones) with a small head-like cluster of hyaline-white conidiospores. These are liberated readily in water or soap water, so I suspect that there is some slime involved. However, at the location where the conidia are present are 3-6 small, flattened plugs projecting out onto which clusters of spores are attached. The spores are subglobose to oval, variable size, possible not hyaline but opaque since spore contents (eg oil bodies) are not seen.
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Hi Thorben, I have found something similar, and I could not pin down the genus, hence posting soon on this forum. Unfortunately, I don't think we have the same organism because mine grows on decaying fruit and the spores are not so hyaline as they dont show the oil bodies.... but is not so different in general. Do you have some key to the genus ?
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Thanks for trying to cheer me up! I am a very emotional Brazilian fan, and I need 3-4 days to be back in the mood. Tomorrow I prepare mulled wine to join you in spirit! (...and do some work on this pluteus).
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It was nice to see again this tiny orange fungus - Pithya cupressina on fallen leaves of Cupressus sempervirens. (Malta, 10-12-22).
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Thank you Harold - later I get grips on Flora Agaracina and dive into the keys. I keep you posted after I recover from mental trauma after my all-time fav. football team got eliminated from the world cup so dramatically - Brazil. I guess many Germans here have passed this already.
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I am working on another new basidiomycete, this time a clear pluteus for its free gills (distant from the stipe), rose-light brown spore print and lack of veil, ring and volva. The light lemon-yellow colours of the stipe and the subspherical spores indicates a P. romelii, but the cream with a very faint hue of yellow colour of the pileus is quite off for the species (P. romelli is caramel to cinnamon brown). So here I am discussing this fungus with you.
Basidia oblongish-subclavate, sometimes flexuous, predominantly 2-spored, rarely 3-spored, up to 35um long
Cheilocystidia are large, lageniform to broad utriform (prone to correction!)
Pleurocystidia present, similar to the cheilocystidia, perhaps a bit smaller
Spores subspherical (some looks spherical) to globose-ovate, Me = 7.1 × 6.3 µm; Qe = 1.1; Ve = 152 µm3; 5.9) 6.2 - 8.1 (8.6) × (5.2) 5.7 - 7.1 (7.7) µm (n=33). In lugol's iodine they showed a dextrinoid reaction, while in Cotton Blue (with lactic acid), the spores collapsed inwards.
They were growing from leaf/twig litter of Eucalyptus plantation. I had in mind Pluteus romellii s.l. and Pluteus boudieri P.D. Orton 1960
I am aware by the work of : Holarctic Species in the Pluteus romellii Clade. Five New Species Described and Old Names Reassessed [ https://www.mdpi.com/2309-608X/8/8/773 ] but all their described species do not show this pale pileus. P. boudieri is my best match so far.
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Spores in 3%KOH (aq) and glycerol (3:1) at x1000 oil immersion.
In my opinion, the single gap in the spore wall is derived from the apiculus. Saying that images of Ph. nemoralis on the web don't show a distinct germ pore, but often you can see 1 germ pore in ten spores. I have photographed some 200 spores, and I can't say I see any distinct examples. Nevertheless, I stick to the keys by Arnolds (2006) and assign this to Ph. vestita. Also, reading the description of Ph. vestita in Arnolds (2006), I see that it is a good match. Moreover, the notes for Ph. nemoralis mention the close relationship with Ph. velata
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I was gonna write earlier that if my specimen had a germ pore, this would perhaps match Ph. nemoralis. What I can do is to mount the spores at x1000 but photography may not be a state of art ! Yet it seems that there is no germ pore (in these images I took).
Pholiotina nemoralis var. nemoralis - PholiotinaPholiotina nemoralis var. nemoralis - inviati in Pholiotina: Pholiotina nemoralis var. nemoralis (Harmaja) Bon, (1991) Basionimo: Conocybe nemoralis…www.ambmuggia.it -
I have found Ph. dasypusa a week or two ago (i think I posted on this forum) and it had a different 'feeling' namely the cheilocystidia were more capitate and less broad, the colours of the pileus not so dark (caramel-brown) with less evident margin veil, and more importantly, the stipe flesh was not reddish brown but pale beige. I think we are not 100% happy with vestita for the striations in the pileus right? --- and the colours are a bit too vivid here.
More cheilos with graphic enhancement - lageniform with long slender necks but variable too.
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Dear friends, I was suspecting that it wasn't a germ pore but in some examples, there was a distinct gap and made me doubt what I am seeing apiculus or a g. pore. That doubt being cleared, the keys of Pholiota in N Europe as per 2006 leaves Ph. vestita as the only candidate. But one must consider what is found in the south, newer species or perhaps something new. I do some more research in this area and see what I find.
here is already something:
Pholiotina vestita - PholiotinaPholiotina vestita - inviati in Pholiotina: Pholiotina vestita (Fr.) Singer, Beih. bot. Zbl. 56: 170 (1936)Basionimo:Galera vestita Fr., in Quélet…www.ambmuggia.itPholiotina vestita - PholiotinaPholiotina vestita - inviati in Pholiotina: Pholiotina vestita (Fr.) Singer, Beih. bot. Zbl. 56: 170 (1936)umbone leggermente più scurowww.ambmuggia.itThank you so much!
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So I am consulting the keys of Pholiotina in Flora Agaricina Neerlandica Vol. 6 (Arnolds, 2005: 180--203).
Our specimen would classify under subkey 2 for its veil present, leaving flocks adhering to the margin
The Cheilo not being capitate would exclude Ph. brunnea.
Now I am not so sure if my spores have a tiny germ pore or not, but if not, then this autumn-fruiting species would be ascribed to Ph. vestita from
the keys mentioned.
If we decide that the spores have a germ pore, P. utriformis is discarded for having utriform cheilocystida
With a spore mean of 8.0 um, the specimens would key to a branch leading to P. velata and P. exannulata. The later should have a pileus with a darker centre when moist , and which my specimens are.
Hmm not really convinced that it is Ph. exannulata though!
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I am glad to have some support re my reasoning of this puzzle. Thank you so much !!!
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Thanks for helping and having an interest in this fungus. I spend a few hours googling and flipping the pages of my microfungi book in vain as well. So maybe I post on ascofrance tmrw and I keep you posted.
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A big thanks to all who contributed. Yes a Hymeniderm 100%. I am quite convinced it is a Pholiotina but I have to go deeper in some key to suggest some options.
Raphael, the shape of the cheilocystidia is shown/documented in my post (if it helps re id)
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Pathyrella clivensis
Psathyrella clivensis Fungus Species
Psathyrella clivensis – Belobetna črnivka – Gobe na Bovškemwww.gobenabovskem.siPsathyrella clivensis - A.M.B. PESAROGenere Psathyrella Specie clivensis (Berk. & Broome) P.D. Orton Sinonimi: Foto Exsic. n. 2815 Erbario Maletti. Determinatore: Maletti M. Foto. Maletti M.…www.ambpesaro.itSpores match incl. size, shape, no germ pore, thick-walled
White Stipe
Short cheilocystidia
No or scanty Veil
Habitat is not so different, also in alkaline soil
Overall colours and small size of cap quite similar